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The agar plates containing TA98 and TA100 were exposed to the following: Aerosol collected matter (ACM) extracted from the e-cigarettes Total particulate matter (TPM) extracted from cigarette smoke Direct cigarette smoke Direct aerosol from E-cigarette Controls: Controls for the particulate test were exposed to Benzo[a]pyrene, 2-aminoanthracene (chemicals linked to cancer), whereas air exposure (no link to cancer) was used as control for the aerosol test

Signs That Weed May Be Hurting Your Lungs Watch for these red flags: Chronic cough that lasts for weeks or months Persistent mucus or phlegm, often worse in the morning Wheezing or a whistling sound when you breathe out Shortness of breath or reduced exercise tolerance Frequent bronchitis or chest infections that linger Chest tightness or pain Asthma symptoms that flare after you smoke (if you have asthma) If these symptoms last more than a few weeks or keep coming back, talk with your doctor about your breathing and lung health